Effect of Using Different Heat Treatments Grass Pea Seed Lathyrus sativa of Secretion on Some Digestion Enzymes of Small Common Carp Cyprinus carpio L.

Abstract

This study was carried out in the laboratories of Fish and Animal Resource Center/Agricultural Researchers Directorate/Technology and Science Ministry to study the excretion of some digestible enzymes for amylase and protease under the effected the of heat treatments and substitute ratio forGrass Pea Seeds Lathyrus sativa (GPS) in common carp Cyprinus carpio L. diets. The experiments of nutrition and digestion were done for 80 days using three different treatments for GPS, roasting, autoclave and cooking. Formulated 13 experimental diets were done as the following diets 1, 2 and 3 used GPS without treatment at ratio 8.25%, 16.75% and 25% from total diet and substitute ratio 33%, 66% and 100% from Soy Bean Meal (SBM), the diets 4, 5 and 6 used roasted GPS at the same ratio and substitute as above. The diets 7, 8 and 9 used cooked GPS at the same ratio and substitute as above and diet 13 for control without GPS. There is a significant difference of digestive enzyme in all studied parts of digestive tract, between after 5-days and 80-days feeding. The activities were (0.45, 0.544, 0.454, 0.454, 0.455 U/M) for amylase and (1.47, 1.49, 1.49, 1.46 and 1.46 U/M) for protease, respectively. The activities of above enzyme after 80 – days feeding trial revealed no significant differences between types of all treatments and the control diets.